01:119:115 Lecture Notes - Lecture 15: Neurospora Crassa, Ribosomal Rna, Archibald Garrod

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Gene expression: the process by which dna directs the synthesis of proteins. Introduction: archibald garrod - found connection between genes and proteins. A. 1. a) those that involved a lack of enzymatic functions (a. 1. a. 1) inborn errors of metabolism. Suggested genes are responsible for phenotypes through enzymes for specific reactions. A. 2. b) genes are responsible for enzymatic function phenotypes. So mendel"s law/principles applies to humans (a. 2. b. 1) genotype dictates phenotypes (a. 2. b. 1. a) i. e. dominant vs. recessive: beadle and tatum, studied neurospora crassa (aka bread mold) Wild type (wt) - requires few nutrients (minimal media) C. 1. a) mold will grow on bare minimum nutrients (c. 1. a. 1) mold with inborn pathways to make other nutrients. Treated fungus with x-rays (high energy radiation [higher than. Conclusions (c. 2. b. 1) 1 gene - 1 enzyme (polypeptic) hypothesis (c. 2. b. 1. a) the function of a gene is to direct production of a specific enzyme (c. 2. b. 1. b) revisions (c. 2. b. 1. b. i) (c. 2. b. 1. b. ii) Not all proteins are a single polypeptide (c. 2. b. 1. b. ii. a) Still not precise: flow of genetic information.

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