BIOL 43800 Lecture Notes - Lecture 2: Safranin, Electron Microscope, Warp And Weft
27 Aug 2018
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Lightmicrosco. pe several dupes compound resolution are close ligh ms. Dark field phase darn image on to separate a bright field small objects that ability together light image dark background contrast can adjust phase. Fwresence microscopy less energy use different stains out than in. 4 not all can get in when cells are dead. Confocal microscopy can look at different planes and highlight. Chemical fixation dyes differential staining gram stain s g acid fast. Gram staining crystal iodine alcohol safranine separates violet as grams and gram primary stain. Storing structures endospore capsule neg stain flagella staining. Stained contrast a vacuum with heavy metals chemical after to scatter to provide fixation. Em vs eight specimine preparation and fix stain chemical negative heavy metal viruses. Shadow cover not high good cryo fixation allows freeze etching tf structures some side with metal and one resolution flagella liquid nitrogen to other see for w structures us freeze the facture along weaknesses sub or carbon noweft.
