BLG 151 Lecture Notes - Lecture 3: Confocal Microscopy, Fluorescence Microscope, Gram Staining
Document Summary
2. 1 lenses and the bending of light. Refraction = a light ray is bent when moving from air to glass. Lenses = focus light rays at a focal point + magnify images. Refractive index = a measure of how greatly a substance slows the speed of light. Focal point = focus light at a single place. Focal length = distance b/w center of lens + focal point. 1 image is enlarged by the objective lens. 1 image is further enlarged by the ocular lens final image. Microscope resolution increases as the wl of light decreases. Max resolution of light microscope = 0. 2 mm. Only uses refracted light to form an image object glow against a dark background. Converts variations in the refractive index + density of cells changes in light intensity, thus colourless, unstained cell = visible. 2 beams of light to create high contrast images of live specimens.