BIOL 2301 Lecture Notes - Lecture 23: Sequence Assembly, Transcriptomics Technologies, Craig Venter
15 Sep 2016
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Genome: all of the genes that make up an organism. Francis collins: led public effort: getting it available to everyone. Craig venter: shotgun sequencing: chop up all dna, put in random primers, let computer sort it out, opinion at time thought it was too hard, it is now the basic principle between large scale sequencing. In 2007, craig venter published his own genome sequence. Model organisms: use in lab to learn about life: apply to human health. Possibility of changing organisms around us for the better. We have no idea where they fit into each other. Genome assembly: use property that if we do this enough, we"ll have overlapping sequences. Computer can then order them together with each other. Get so many reads that you get lots of overlap. Generate sequences- lined up to form contigs. In order to identify disease genes and to determine what makes each person different thousands of human genomes are now being sequenced.
