BIOL-UA 21 Lecture Notes - Lecture 24: Contig, Tata Box, Human Genetic Variation
22 Dec 2016
School
Department
Course
Professor
Document Summary
Lecture 24: sequencing: genomics: study of the structure, function and evolution of genomes, chemical sequencing, sanger sequencing, human genome project, and celera genomics corporation collaborated and sequenced the entire genome, assembling human genome sequences: Find complete sequence of a,c,g and t in dna of an organism. There is no machine that takes long dna as an input, and gives the complete sequence as output. Can only sequence 500 letters at a time: strategy for human genome project: Small dna fragments into bacterial arti cial chromosomes (bacs). Cut up dna into 100-300 kb fragment and cloned into bacs. The bacs to be sequenced were fragmented into 1 kb shotgun clones and sanger sequencing was performed. The sequences were aligned and the human genome was generated: bac library: Make a bac library to cover entire genome. You want to include every genomic region, and some genomic regions will be covered by more than one bac: minimizing number of bacs to sequence:
