BIO 350 Lecture Notes - Lecture 9: Nucleoplasm, Enzyme, Nuclear Dna
Document Summary
Initiation: elongation, termination, same three main steps but the mechanisms and number of players are more diverse. Before initiation can occur the chromatin must be remodeled - why: chromatin is tightly packaged dna. The package must be opened prior to initiation: open chromatin is necessary but not part of the transcription, not to be confused with bacteria"s open complex formed by rna polymerase holoenzyme (a2bb"ws). Trans-acting elements assist in opening chromatin: remodeling factors can create a gap between dna and the histone protein. How does a eukaryotic rna polymerase find its promoter: basal transcription factors are responsible for reading the nuclear dna and finding the cis elements. The basal transcription factors are required: transcription factors along with partner proteins are responsible for regulating the assembly of the transcriptional apparatus. What is a transcription factor: protein, can bind dna, sequence specific, refulates transcription.
