ECON 1 Lecture Notes - Lecture 2: Kallmann Syndrome, Viability Assay, Signal Transduction
Document Summary
Fgf signalling complex: 2 receptors coming together through two ligands, they give a signalling complex, tyrosine kinase is auto phosphorylated. Proteins: fgf (fibroblast growth factor, e-selectin (binds to fgfs, cadherins (binds to fgf receptor and signals, neuropilin-1 (increases cell growth response, anosmin-1 (alters cell growth response) Proteoglycans: heparin and/or hs (heparin sulphate) glucosaminoglycans (carbohydrates, specialised glycoprotein family. Heparin sulphate proteoglycans structure and function: family of complex sulphated polysaccharides attached to a protein core, strategic expression on cell surfaces and within the extracellular matrix. Multifunctional cell regulators: they regulate functions of many proteins via specific carbohydrate sequence motifs. Hs sequence dictates the overall conformation of a polymer: confers selective protein-binding properties. C h l o r a t e t r e a t e d mi n. Normal cells: baseline, after 120 minutes, the lines fade: fgf2: increase in tyrosine kinase activity, fgf2 with heparin: required to promote strength at that period, hs regulates the kinetic signaling.
