ECON 1 Lecture Notes - Lecture 26: Enterocyte, Alternative 3, Eif4Ebp1
Document Summary
Rrna: phylogenetic studies rrna sequences highly conserved across species. In vitro translation systems used when overexpressed protein is toxic, rapidly degraded or insoluble in cells: mutations in mitochondrial rrna susceptibility to aminoglycoside-induced hearing loss. Canonical nmd pathway: mammalian cells: upf1 and smg1 (kinase) bind to erf1 and erf3 release factors (eukaryotic) to form the surf complex near the. Upf3b and ejc (exon junction complex) downstream of the ptc triggers formation of the decid complex: changes to rrna in cytoplasmic ribosomes defective, results in upf1 phosphorylation and pseudouridylation and cross-linked dyskeratosis. Upf1 (up-frameshift suppressor 1 homolog; key factor for nmd, a dna/rna helicase: cbc initiates the pioneer round of translation and binds upf1. Control of translation: global all mrnas throughout the cell, temporal - specific mrnas in response to specific cues, positional in specific positions within the cell. Ribosome profiling: method to study control of translation and in vivo.
