BIOL 5060 Lecture Notes - Lecture 24: Omics, Chromatin, Reverse Transcriptase
Document Summary
Approach with a transgene- how do we get it expressed in a specific tissue: 1. Use a gal4 driver reporter system- transcriptional activator from budding yeast. We will take the transgene and its promoter with a series of gal4 binding sites (that we"ve added) We have turned it into a gal4 regulated promoter (mouse a); reporters- respond to different drivers. We have tissue specific promoter expressing gal4 (mouse b): drivers. We make the reporter and purchase a bunch of different drivers. We cross these mice and we have a double transgenic progeny . Now we have gal4 only expressed in a specific tissue and the transgene only expressed in a specific tissue***** Approach with a conditional knockout- still using gal4 driver system: cre recombinase (protein) and loxp sites (sequence, how cre works. We will get: a loxp c: it will eat up everything between the loxp sites, so lets say we have a goi.
