BIOL 5060 Lecture Notes - Lecture 25: General Idea, Chromosome, Sister Chromatids
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2) synthetic lethal screen (previously discussed)- a way of starting with mut1- and looking for mut1-mut2- Systematic gfp fusions- global localization of protein: allows for quantitation- how many molecules of protein per cell under various conditions. Systematic tap tags- allows for affinity capture (purification) of all proteins to identify members of protein complexes. In vitro transcription translation- trying to make a specific labeled radioactive protein: to make radioactive molecules of two proteins, transcription will produce rna that encodes protein x (the only. Rna hammerhead- how can you reduce activity; morpholines (modified rna molecules that can"t get chewed up or antisense will knock down translation: but antisense with hammerhead will not only knock down translation, but destroy the transcript, design antisense. Rnai mechanism- rna interference; take long dsrna and a dicer chops it up into short (21 bp) fragments rits complex to shut down gene expression cleavage: not conditional; any tissue expressing the transcript will be effected.
