BIOL 2040 Lecture Notes - Lecture 11: Transfer Dna, Recognition Sequence, Ecori
Document Summary
This was perhaps the first method used to follow segregation of dna directly. Below, these are based upon use of pcr - the polymerase chain reaction. Example ecori has a recognition sequence of gaattc . Hybridize probe to membrane and expose to xray film. First pcr amplify gene or dna of interest, then digest with restriction enzyme, then run on agarose gel and stain gel. from all individuals. An example of caps for a test cross. In principle this method can detect two alleles (or portions thereof) if they differ by at least one base pair. Pcr ampify the gene of interest from each genotype. Denature the dna by heating and cool rapidly so it remains single stranded. Since dna is single stranded, under the particular gel running conditions, the single stranded dna folds up depending on its sequence, and then runs through the gel as a function of the way it has been folded.
