BIOL 2040 Lecture Notes - Lecture 6: Molecular Marker, Haplotype, Restriction Fragment Length Polymorphism
Document Summary
Representation of all chromosomes in the genome as lines, marked with the positions of genes known from their mutant phenotypes, plus molecular markers. Cis-conformation in a heterozygote, having two mutant sites within a gene or within a gene cluster, the arrangement a1a2/ a1a2. The ratio of the observed number of double recombinants to the expected number. Crossing over the exchange of corresponding chromosome parts between homologs by breakage and reunion. Crossover product meiotic product cells with chromosomes that have engaged in a crossover. The autoradiogrpahic banding pattern produced when dna is digested with a restriction enzme that cuts outside a family of vntrs (variable number of tandem repeats) and a sothern blot of the electrophoretic gel is probed with a vntr-specific probe. Unlike true fingerprints, these patterns are not unique to each individual organism. A distance on the chromosome map corresponding to 1% recombination frequency.
