BIOL 2040 Lecture 6: BIOL 2040 Lectures 6-8.PDF

For each of the following, decide whether RNA cleavage, Inhibition of translation or transcriptional silencing is involved and why.

i) a miRNA/RISC complex binds to mRNA.
ii) siRNA/RISC complex performs Slicer activity
iii) Complex with siRNA attracts DNA methylating enzymes.

Which enzyme processes double stranded RNA into short interfering RNAs of approximately 21 nucleotides in length?

Explain the following aspects of miRNA biosynthesis: A. Pri-miRNA B. Pre-miRNA C. Drosha D. pasha E. Exportin -5 F. Dicer G RISC H. Argonauts I miRNA Complex J. miRNA and active RISC