BIOL 1000 Lecture Notes - Lecture 18: Electron Transport Chain, Electrochemical Gradient, Citric Acid Cycle

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BIO 1000: Cellular respiration
Glycolysis split sugar into 2 three carbon molecules. Net generation of ATP
Pyruvate oxidation trasitio etee glyolysis ad ker’s yle. Pyruate oerted ito 
acetyl-CoA (net of 2). 2 NADH produced and 2 CO2 released.
Kre’s Cyle – complete oxidation of glucose. Generate a bit of ATP and electron carriers
Electron Transport Chain transferring protons (H+) from the matrix to the intermembrane
space creating the proton concentration gradient.
Chemiosmosis bulk of ATP generation. Harnessing energy present in proton gradient to drive
chemical reactions
In cellular respiration we make a total of 10 NADH and 2 FADH2
for eah NADH e get  ATP’S
for each FADH2 e get  ATP’S
So 10 x 3 = 30 ATP
2 x 2 = 4 ATP
Add that up and you get 34 ATP from chemiosmosis
So the net ATP production is then 34 ATP from oxidative phosphorylation
2 ATP from glycolysis
 ATP fro the to turs of the Kre’s yle
which gives us a grand total of 38 ATP
you must invest 2 ATP
Per turn of krebs
o ATP 2
o FADH2 2
o NADH 6
Electron transport chain inner membrane
o Proton gradient
o NO ATP MADE IN ETC
Complex 1 = dehydrogenase oxides NADH (moving of hydrogen)
Complex 2 = dehydrogenase
Comples 1 & 4 = allow movement into intermembrane space
Ubiqinone shuttle moving from one complex to the next
Energy releases as you move from complex to complex
Electron flow goes from high to low electron energy
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Document Summary

Glycolysis split sugar into 2 three carbon molecules. Pyruvate oxidation tra(cid:374)sitio(cid:374) (cid:271)et(cid:449)ee(cid:374) gly(cid:272)olysis a(cid:374)d ker(cid:271)"s (cid:272)y(cid:272)le. Pyru(cid:448)ate (cid:272)o(cid:374)(cid:448)erted i(cid:374)to (cid:1005) acetyl-coa (net of 2). Generate a bit of atp and electron carriers. Electron transport chain transferring protons (h+) from the matrix to the intermembrane space creating the proton concentration gradient. Harnessing energy present in proton gradient to drive chemical reactions. In cellular respiration we make a total of 10 nadh and 2 fadh2 for ea(cid:272)h nadh (cid:449)e get (cid:1007) atp"s for each fadh2 (cid:449)e get (cid:1006) atp"s. Add that up and you get 34 atp from chemiosmosis. So the net atp production is then 34 atp from oxidative phosphorylation.

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