Microbiology and Immunology 2500A/B Lecture Notes - Lecture 3: Paramyxoviridae, Reverse Transcriptase, Poxviridae
29 Apr 2021
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Document Summary
It was established that the viral genome is the genetic code in the 1950s. Nucleic acid or protein (shell: allow bacteriophage to infect with radioactive precursors to dna or to protein, blend , separate , radioactive phosphorus could be detected in next generation of bacteriophage. Modern-day hershey-chase experiment: proof the genome is key. Nucleic acid or protein (shell: bacteriophage mixed with cyanine dye (binds viral genomes, upon infection into bacterial host, the dye leaves bacteriophage and enters host interior. Key concept: viral genomes must make mrna; this mrna must be read by the host ribosomes mrna is read in the 5" to 3" direction - there are no exceptions yet!! Viral genomes must make mrna; this mrna must be read by host ribosomes. However, viruses do not do this in a linear fashion (no dogma!) Common goal: production of mrna to be translated by host cell machinery. Mrna: by convention is the + strand; is ready to be translated.
