Microbiology and Immunology 2500A/B Lecture Notes - Lecture 22: Baltimore Classification, Cyanine, Rna Virus
15 Jan 2017
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Document Summary
Remember to talk about physical vs. infectivity. Attach a gene that codes for the fluorescent protein to the virus of interest. This allows you to see which cell has been infection with the virus (will be fluorescent) Understanding the various types of genomes found in viruses and how these genomes function. Background on the importance of the genome. Viral classification scheme using the baltimore method. How dna based genomes get their message across. How rna based genomes get their message across. Genome allows for synthesis of required elements. Virions are vehicles to transmit virus genome to the next host. 1950 experiment: established that viral genome is the genetic code (genome is key) Is the the nucleic acid (genome) or the protein (shell) Wants to prove: for a virus, its genome is key for transmission and replication. Ran parallel experiments using bacteriophage (virus) that was allowed to infect e. coli. In one experiment, the shell was radioactively labelled.
