Biology 1202B Lecture Notes - Lecture 16: Electrophoresis, Sticky And Blunt Ends, Dna Profiling
11 Apr 2016
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Document Summary
Dna technologies often use "optimised" enzymes or "complementarity" of dna/dna and. Require a sufficient amount of dna of "interest". o. To attain a sufficient amount of specific dna, bacterial plasmids are utilized. Bacteria can replicate (inserted) plasmids very rapidly when given the proper resources (nutrients) Restriction enzymes: cut dna at a specific sequence (typically for virulent or foreign dna) o o. These enzymes don"t digest their own dna due to epigenetic "markers" & methylation. Cutting: restriction enzymes often cut the dna by recognizing 4-6bp and cut either straight down the middle or unevenly. Sticky ends: any piece of dna cut by the same restriction enzyme can be. "stuck back together" as the sticky ends have complementarity. Cut the plasmid and the dna of interest with the same restriction enzyme and. "stick back together" and seal the sugar-phosphate backbone with dna ligase creating recombinant dna. Digest dna of interest with a restriction enzyme.
