Biology 1002B Lecture Notes - Lecture 18: Viral Vector, Intron, Transfer Rna
31 May 2016
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Document Summary
Optogenetics provides control of specific neurons at specific times in behaving animals. Action potentials are propagated by movement of ions across membranes: inside of nerve is highly negative. Nerve membrane can be permiabialized (depolarize a cell to conduct a message) Action potentials can be controlled by channelrhodopsin from chlamy: chlamy channelrhodopsin in neuron of a mouse. Blue light opens the channel, depolarizing the neuron: we can also prevent depolarization. How can you get expression of channelrhodopsin into a specific gene: recombination restriction enzymes. 4-cutters // 6-cutters (number of bases in restriction site seq. : (cdna) genes can be created by reverse transcription of mrna, dna printer can create a sequence. How do you get the gene into the mouse: vector using plasmid, put recombinant plasmids into bacteria. Need genes to be expressed not inside: ampr gene selectable marker ampicillin is an antibiotic, bacterium needs the gene so its is not killed. Eukaryotic proteins produced in a prokaryotic cell.
