Biology 1002B Lecture Notes - Lecture 20: Comparative Genomics, Synteny, Intron
7 Apr 2016
School
Department
Course
Professor
Document Summary
Determines base by determining what nucleoides are needed in order to replicate the strand. Dideoxynucleoides are missing the oh (replaced by h) Without oh, dna polymerase can"t add another nucleoide to the strand and producion stops (creates fragments) Built with about 1% dideoxy nucleoides and the rest normal. Creates tons of iny strands difering by a length of one nucleoide. Run through electrophoresis to separate strands (diference by a length of one nucleoide shown on gel) 4 diferent well plates for each type of dideoxynucleoide or 1 well using a laser and luorescence tags to determine the base added. Dna synthesis releases pyrophosphate (can use atp which generates light) Pysosequencing processes many diferent fragments at one ime. Pcr is used to amplify the fragment stuck on each bead (thousands of the same fragment on one bead) -> don"t need to know how. If atp is incorporated, you will get a lash of light.
