Biology 1002B Lecture Notes - Lecture 10: Optogenetics, Mental Floss, Channelrhodopsin
3 Feb 2020
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Start w double stranded sequence, make it single stranded, and add a bunch of promoters. Dna primers that we synthesize with a gene machine dna synthesizer and then use taq pol to extend these primers. The primers make pcr specific, the taq pol is used because it remains functional at high temps. We separate the two backbones by increasing the temperatures. You need a pol that will not denature at high temperatures. By the 3rd cycle we start toget exact copies of original sequence and then they increase exponentially. We can get billions of copies of specific sequence. Slide 4: various technologies deliver transgenes to nuclei or organelles. We can get any gene we want to into any organism we want to. Vectors: expression controls, start and stop primer, selectable marker, may also have a reporter gene. Slide 6: optogenetics provides control of specific neurons at specific times in behaving animals.
