Biology 1001A Lecture Notes - Lecture 5: Exonuclease, Thymidine Triphosphate, Nitrogenous Base

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12. 2a watson and crick brought together information from several sources to work out dna structure. Before watson and crick, investigators established dna contains 4 nucleotides, each of which consists of the 5-carbon sugar deoxyribose, a phosphate group, and a nitrogenous base (a,g,t,c) Adenine and guanine are purines (built from a pair of fused rings of carbon and nitrogen atoms) Thymine and cytosine are pyrimidines built from a single carbon ring. The amount of purines equals the amount of pyrimidines: a=t (amount, c=g (amount) Dna has a sugar (deoxyribose)-phosphate backbone known as a polynucleotide chain: the connection between adjacent deoxyribose sugars is a phosphodiester bond, the chain has polarity. 5" end a phosphate group is bound to the 5" carbon of a deoxyribose sugar. 3" end hydroxyl group is bound to the 3" carbon of a deoxyribose sugar. The number of polynucleotide chains in a dna molecule and the manner in which they fold or twist were unknown at the time.

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