Biology 1001A Chapter Notes - Chapter 12.3-12.4: Base Pair, Phosphodiester Bond, Dna Clamp

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12. 2 a) watson & crick works out dna structure. Other investigators had already established the fact that dna contains 4 different nucleotides (atcg) Each nucleotide is made of deoxyribose (a 5c sugar: each deoxyribose is #ed from1" to 5", carries a phosphate group, carries 1 of 4 nitrogen bases (a,g,c,t) Purines have nitrogenous bases built from 2 fused rings of carbon & nitrogen: adenine (a) and guanine (g) Pyrimidines are build from a single carbon ring: thymine (t) and cytosine (c) Chargaff"s rule: amount of bases occur in de nite ratios, # of purines = # of pyrimidines; # of a = # of t; # of g = # of c. The 2 ends of a polynucleotide chain are different, one end is 5" while there other is 3". Franklin used x-ray diffraction is to determine the helical structure of dna: dna molecules are cylindrical, 2nm in diameter, major patterns repeat every.

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