Biology 2382B Lecture Notes - Lecture 19: Prenylation, Acylation, Glycosylphosphatidylinositol
24 Mar 2017
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Membranes are lipid bilayers: outer/inner leaflet, cytosolic leaflet, exoplasmic leaflet. Membrane fluidity 2 d (lateral) movement: almost nothing moves leaflet to leaflet (flip-flop) Lateral fluidity depends on: fatty acid composition, protein fluidity in membrane contributes to overall fluidity of membrane, longer fatty acids (higher melting temp) = less fluid, shorter fatty acids (lower melting point) = more fluid. Cholesterol: proteins when linked to cytoskeleton, will limit fluidity around. Frap fluorescence recovery after photo bleaching: all cells have lots of proteins that can be made fluorescent (gfp) Protein fluidity: outside membrane glows, photo bleach an area (takes away fluorescence, check for fluorescence recovery after photo bleaching, will recover if proteins outside bleached area migrate over. If proteins diffuse laterally fluid: graph: certain amount of fluorescence, bleach = decreases fluorescence, recovery phase (in ex. 50% recovery: means 50% protein were fluid (mobile, 50% proteins connected to cytoskeleton (immobile, normally level always drops below original after bleaching (always non-mobile proteins)
