Biology 2382B Lecture Notes - Lecture 2: Chromophore, Plasmid, Beta Barrel
Document Summary
First microscope was one lens and put up to the light, first person to look at molecules. 200x magnification due to lens he made. Too see detail in specimen we need contrast which is created from the light source. Contrast is the ability to bend light. Things with higher refractive index (can bend light more) are easier to see. Total magnification is objective lens magnification + the ocular magnification. 1000x is max magnification in modern compound microscope. Resolution is the ability to see 2 objects as 2 distinct objects. how close can you bring two objects together but still them as two entities. We (cid:272)a(cid:374)"t see (cid:448)iruses (cid:449)ith (cid:373)odern compound need electronic. We need to know equation to understand how microscopes work. Alpha is 1/2 angle of light entering the objective lens, the closer you can get the object to the lens the better. When achieving optimal resolution, we want low d # = high resolution.