Biology 2382B Lecture Notes - Lecture 2: Differential Interference Contrast Microscopy, Phase-Contrast Microscopy, Fluorescence Microscope
11 Feb 2017
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First to observe living protazoa and bacteria which he called (cid:498)animalcules(cid:499: used glass, water, brass plate, put the specimen into the water and put it to sunlight. Went on to visualize human red blood cells and sperm. With great skill at grinding lenses, naturally acute eyesight and lots of patience he was able to achieve a magnification of 200x: magnifying properties and could see certain detail, primitive microscope. Not a single lens, more than one (unlike anton: ocular and condenser lens = compound microscope (more than 1 lens) Ocular or eyepiece lens: focus image onto eye. Typical light microscope magnification is 40 to 1000x. Total magnification is the product of the magnification of ocular and objective lens: 100x objective lens does not mean 100x magnification. What you see is that multiplied by the magnification of the ocular lens: ocular lens: 10x magnification. 1000x is the maximum magnification in a conventional light microscope.
