Biochemistry 3385A Lecture 16: Lecture 16 – Repair Diseases and Mechanisms
4 Mar 2015
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This nucleoprotein filament searches for homologous duplex dna, and a strand exchange reaction generates a joint molecule between the damaged and undamaged dna. c) dna polymerases and their associated factors carry out repair synthesis and holliday junctions are formed. d) holliday junctions are resolved by endonucleolytic cleavage and rejoining in a reaction that may involve the mus81 protein and in which two intact dna molecules are formed. Non homologues end joinging a) dsbs are recognized by the ku complex (ku70 and ku80 proteins) which specifically bind to dna ends and then recruits the dna dependent protein kinase catalytic subunit (dna pkcs). b) the ends of the break are processed if needed in a step involving artemis and potentially other nuclease(s); a few base pairs may be removed in this step. c) the two ends are ligated by xrcc4/dna ligase iv to restore a continuous dna molecule.
