BIO 3170 Lecture Notes - Lecture 6: Rad51, Non-Homologous End Joining, Reca
10 Oct 2016
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Document Summary
Major pathways of dna double strand break repair vs recombination. Loss of materials-product shorter than initial dna piece. 2 strands damaged, other chr. used for repair. Copying mechanism regenerates dna - before damage: homology directed repair. No transfer of sequences: recombination physical transfer of genetic material in between 2 strands. Repairing double strand breaks - no intact complementary strand. Non-homologous end-joining (nhej) alters the original dna when repairing broken chromosomes. Homologous end joining = no loss of dna copy sequence of another chromosome and insert it: homologous end-joining is harder to perform but more precise, occur in various circumstances: ionizing radiation, oxidizing agents, replication errors. Initiate the process of homologous recombination: most important nhej. Or base-pairing between short regions of homology loss of dna sequences: trimming of single stranded tails and ligation. Ku recognize and bind free ends of dna where break is.
