Biochemistry 2288A Lecture Notes - Lecture 22: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Gel Electrophoresis

• School: Western University
• Department: Biochemistry
• Course: Biochemistry 2288A
• Professor: Chris Brandl

Document Summary

Or make thing more efficiently such as detergents: environment (bioremediation). Example: bacteria that eat oil: forensics and law: dna fingerprinting, case study: you have identified a human protein that will be of tremendous value. You goal is to mass produce this gene. Only for a few genes that lack introns. Dna denaturation: heat or add a base the strands come apart. Melting temperature or tm: the temperature at which 50% of the molecules are single stranded. This is a combination of molecules not just one. Intrinsic features of the molecule itself: the more g:c content the higher the tm (3 h-bonds, the longer the dna molecule the higher its tm, dnas that have the right base pairing have a higher tm compared to. Dnas with a single base mismatch: extrinsic factors that affect the tm, salt concentration: the higher the salt concentration, the higher the tm.