MGY377H1 Lecture Notes - Lecture 15: Histidine Kinase, Methyl Group, In Rainbows
1 Feb 2018
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They looked at the amino acids in contact, and they changed some residues and changed the interactions. It normally signals via envz and it results in phosphorylation. The kinase phosphorylates itself and it then phosphorylates things downstream. They then made a series of mutants which make envz look more like rstb. They sequentially added residues from rstb and put them into envz. We only need to make 3-4 amino acids changes in the histidine kinase to make it signal into a different system. Thus the way these systems are built and since they evolve from eachtoher, it does(cid:374)"t take (cid:373)a(cid:374)(cid:455) (cid:272)ha(cid:374)ges i(cid:374) a histidi(cid:374)e ki(cid:374)ase to (cid:373)ake it do so(cid:373)ethi(cid:374)g else. We are trying to understand how bacteria feel their environments. Bacteria have signaling pathways, and we are interested in how these signaling pathways which are proteins interacting with eachother sense the environment. Bcteria have 4 genes to correct these mutations.
