IMM250H1 Lecture : Notes taken during lecture
Document Summary
Ig like domains are frequently seen in otehr types of molecules - not limited to immunoglobulins. Interactions btw antibody and antigen and how this intearction based on recognition of epitopes on surface of anitgens by antibodies. Affinty of this interaction no covalent bonds. Affinity single epitope and single antigen binding site of antibody. Avidity valence of interaction btw antigen and antibody. Different isotypes of antibody kappa versus lamba light chains; two distinct light chain types functionally equivalent. Classes and subclasses have differing functions, evolved different functions. Allotypes genetic polymorphisms btw individuals when considering particular class or subclass of antibody. Ideotypes markers of immunoglobulin b region domains light and heavy chains. Southern blot of dna from germ line sperm and identified genetic fragment that corresonded to constant region, and anotehr to the v region. With restriction enzymes, specific sizes of fragments in germ line dna. Constant regions the same with b cell, v region different different restriction enzyme sites.
