HMB265H1 Lecture Notes - Lecture 10: Protease, Signal Peptidase, Glycosylphosphatidylinositol

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HMB265H1 Full Course Notes
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HMB265H1 Full Course Notes
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Bio230 lecture 10 - protein sorting (oct. 10) How proteins make it into and out of nucleus of eukaryotic cell. Specific example: nfat (transcription factor that shuttles into and out of nucleus) All proteins of eukaryotic cell synthesized and translated in cytosol. = have to go to different compartments co-translationally or post-translationally. Gated proteins: cytosol to nucleus, nucleus to cytosol. = protein complexes (usually made up of multiple proteins) that move proteins across membranes. Transport proteins across membranes (from cytosol across membranes that envelope these organelles) Contrast with gated transport proteins that are usually folded already. Mitochondria and chloroplasts have their own genomes. = have own genomes/ribosomes so can function like own entity. But most proteins found in mitochondria and chloroplasts are nuclear encoded. = most proteins still encoded in nucleus, translated in cytosol and have to get into mitochondria/chloroplast. Movement of proteins into these organelles occur in a post-translational manner, proteins unfolded.

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