BIO230H1 Lecture Notes - Lecture 4: Tata Box, Non-Coding Rna, Eukaryotic Transcription
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BIO230H1 Full Course Notes
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Document Summary
Cells produce several types of rna: prokaryotes and eukaryotes: Mrna trna rrna: only eukaryotes: snrna sirna scarna snorna. Different rna transcribed by different rna polymerases in eukaryotes: rna polymerase ii mrna. Prokaryotes have single type of rna polymerase. Transcription initiation in eukaryotes requires many proteins general transcription factors: help position rna polymerase at eukaryotic promoters containing tata box, tfiid. Taf subunits: recognize other dna sequences, regulates dna binding by. Phosphorylates ser5 of rna polymerase"s ctd tail. Releases rna polymerase from promoter: required by nearly all promoters used by rna polymerase ii. Eukaryotic transcription: rna polymerase ii transcribes protein coding genes, requires 5 general transcription factors: tfiid, tfiib, tfiif, tfiie, tfiih. Activator influences mediator which influence all other proteins to start transcription; cis regulatory sequence = site of activator binding. Eukaryotic gene expression controlled by many regulatory proteins encoded by human genome (~2000) both activators and repressors.
