BIO130H1 Lecture Notes - Lecture 18: Translocon, Digestive Enzyme, Endosome
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BIO130H1 Full Course Notes
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Golgi apparatus: endo/lysosomes, protein sorting, plant vacuoles, peroxisomes. Signal seq at the amino-terminal end directs protein to er. Talks about the first case on the last slide (signal sequences) Signal seq cleaved, left behind in er membrane, secreted protein ends up in er lumen. Secreted protein moves in transport vesicles via secretory pathway. Below: either you start at (1) and go to (2) and (3) Or (1) then (2a) and (3a) - two separate pathways. Secreted proteins move into er lumen for example. The hydrophobic transmembrane segment is the second case on signal sequences. Where the sequence is in the middle of the protein. Checks the neighbouring amino acids to the segment. Check which side is more neg and which is more pos, relatively. Case 1: er lumen side is more negative (n-terminus) Translocon protein will have opposing charges, so it stays. N-terminus stays in the er lumen and c face to the cytosol.
