BIO130H1 Lecture Notes - Lecture 2: Lipid-Anchored Protein, Glycosylphosphatidylinositol, Crystallography
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*they rarely flip-flop (moving from one leaflet to another leaflet) on their own. However, phospholipid-translocator (a unique enzyme(protein)) help the flip-flop of lipids for synthesizing a new membrane. 1. temperature (very important) (in low temp-rigid, gell like) By observing the artificial bilayer lipids, we can determine the temperature by the fluidity of the membrane. The presence of cis-double bond (unsaturated, has kinks) allows to remain flow easily in low temp. Shorter tials increase the fluidity of the membrane in a low temp. The composition of phospholipids in the plasma membranes of two different single-celled organisms isolated from the ocean are examined. Organism a was isolated from the water surrounding a thermal vent where the temperature was 80oc. Organism b was isolated from the ocean floor where the temperature was 4oc. A. organism b will have more saturated phospholipids that also contain shorter hydrocarbon tails. (b is in lower temp, so it should have unsaturated phospholipids!)
