BIO130H1 Lecture Notes - Lecture 5: G2 Phase, G1 Phase, S Phase

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Cell division observed in a lung cell maintained in cell culture- very few cells can be observed in the process of cell division at any given time. M phase: the nucleus and cytoplasm divide, includes mitosis and cytokinesis (cytokinesis is nuclear division and cytoplasmic division). Interphase: the period between cell divisions, includes the g1 phase, the s phase (dna replication occurs in s phase, long before mitosis) and the g2 phase, these are the steps a cell undergoes in preparation for mitosis. Cells that do not divide are in g0: Cells are not dividing but it doesn"t mean they are dying. They can still transcribe and translate, but they are not undergoing mitosis. Some of these cells (with the right stimulus) can go back into the cycle by starting at g1. They then move into s, then g2 then mitosis (m) and then undergo cytokinesis. Some cells will go to g0, some will exit the cycle.

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Part A:

In prokaryotes and eukaryotes cell reproduction is similar in that it requires some kind of signal to proceed, DNA replication, careful separation of the DNA into two equal portions, and division of the cytoplasm. How is cell reproduction different in prokaryotes and eukaryotes?

Select one:

a. In a eukaryotic organism, the signals for cell division are usually not related to the environment but to the function of the entire organism.

b. Eukaryotic cells constantly divide whenever environmental conditions are adequate and the cells are large enough.

c. The linear chromosomes in prokaryotes are constantly being updated.

d. Prokaryotic cell division requires cytokinesis before mitosis because of their small size.

e. The sexual phase of most prokaryotes (bacteria) is essential to the rapid cell division required for infection.

Part B:

What does the name Cyclin Dependent Kinase stand for?

Select one:

a. A protein important to the cell cycle that is dependent on DNA synthesis.

b. An enzyme that cuts DNA so that chromosomes can segregate.

c. An enzyme that phosphorylates other proteins using ATP only when the protein Cyclin is bound to the enzyme.

d. An enzyme that rolls along microtubules dragging a vesicle.

e. An enzyme that connects chromosomes to the spindle fiber.

Part C:

The Cell Cycle is divided into Mitosis, Cytokinesis, and Interphase. Mitosis can be ordered into the following phases:

Select one:

a. Prophase/Prometaphase/Metaphase/Anaphase/Telophase

b. Anaphase/Prometaphase/Metaphase/Prophase/Telophase

c. Metaphase/Anaphase/Telophase/Prophase/Prometaphase

d. Telophase/Prophase/Prometaphase/Metaphase/Anaphase

e. Anaphase/Telophase/Prophase/Prometaphase/Metaphase

Part D:

The Cell Cycle is divided into Mitosis, Cytokinesis, and Interphase. Interphase can be ordered into the following phases:

Select one:

a. S/G1/G2

b. G2/G1/S

c. G1/S/G2

d. G2/S/G1

e. G1/G2/S

amethystyak657

Question 3 (5 points)

Match the terms to the definitions provided.

Question 3 options:

	telophase II
	meiosis II
	telophase I
	anaphase I
	anaphase II

1.	Division, beginning without DNA replication, that resembles and produces four haploid cell.s
2.	Phase of meiosis when chiasmata are broken as spindle fibers shorten and homologous pairs are pulled apart.
3.	Phase of meiosis when sister chromatids are pulled to opposite poles of the cells.
4.	Phase of meiosis in which homologues cluster at the poles of the cells and the nuclear envelope reforms.
5.	Phase of meiosis in which four different haploid cells are present.

Question 4 (7 points)

Match the terms to the definitions provided.

Question 4 options:

	G0
	telophase
	G2
	mitosis
	cytokinesis
	cell cycle
	S

1.	The phase in which the cell synthesizes a replica of its genome
2.	The overall process of genome duplication
3.	The phase in the cell cycle during which mitochondria and other organelles replicate.
4.	The phase in mitosis when chromosomes are clustered at opposite poles and the spindle apparatus disappears
5.	A resting state that cells often enter before resuming cell division.
6.	The phase in the cell cycle when the cytoplasm divides creating two daughter cells.
7.	The phase in the cell cycle in which the spindle apparatus assembles and sister chromatids move apart.

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Question 5 (6 points)

Put the events of a MAP kinase cascade in the correct order.

Question 5 options:

	MAPKKK phosphorylates and activates another kinase
	MAPKK phosphorylates and activates MAPK
	Binding of MAPKKK to Ras
	External signal binds to membrane receptor and activates receptor kinase.
	MAPK phosphorylates an inactive response protein and activates it.
	Phosphorylation of receptor kinase and interaction with RAS

M2A1 Worksheet: Mitosis and Meiosis

A dog is a diploid organisms and has 39 pairs of chromosomes (2n = 78), where n = 39 chromosomes from each parent. Use this example to answer the following question regarding chromosomal number.

INTERPHASE

How many chromosomes are present in the somatic cell?

What do they look like?

What are they âdoingâ in G1 of this phase?

What is happening in the S phase?

What is happening in the G2 phase?

What follows G2 phase in a somatic cell?

What follows G2 phase in a gamete cell?

MITOSIS

Number of chromosomes in the parent cell.

Number of chromatids in the parent cell.

Number of chromosomes in the daughter cell.

Number of chromatids in the daughter cell.

How many daughter cells are produced?

Are the daughter cells haploid or diploid?

How many cell divisions take place?

In what type of cell does this division occur?

Meiosis 1:

Number of chromosomes in the parent cell.

Number of chromatids in the parent cell.

Number of chromosomes in each Meiosis I daughter cell.

Number of chromatids in each Meiosis I daughter cell.

Are the daughter cells haploid or diploid at the end of Meiosis 1?

Meiosis II:

Number of chromosomes in the âparentâ cell (which is a daughter cell from Meiosis I).

Number of chromatids in the âparentâ cell.

Number of chromosomes in each Meiosis II daughter cell.

Number of chromatids in each Meiosis II daughter cell.

Are the daughter cells haploid or diploid at the end of Meiosis II?

Which phase or phases of Meiosis account for the genetic variability among the daughter cells? Howâ¦ what happens in this phase to create the diversity?

Comparing Mitosis and Meiosisâ¦..

How many daughter cells result from Mitosis? Are they diploid or haploid?

How many daughter cells result from Meiosis? Are they diploid or Haploid?

Cell structures and function: Match each organelle to its function:

Organelle Function

Lysosome 1. Lipid synthesis

Rough ER 2. Houses DNA

Nucleus 3. Energy extraction

Smooth ER 4. Dismantles debris

Golgi apparatus 5. detoxification

Mitochondria 6. Protein synthesis

Peroxisome 7. Processes secretions

What is the function of checkpoint proteins in the cell cycle?

How can all of a personâs cells contain exactly the same genetic material, yet specialize as bone cells, nerve cells, muscle cells and connective tissues?

What are the 3 primary germ layers?

Briefly, in one or two sentences, distinguish between

a bacterial cell and a eukaryotic cell

interphase and mitosis

mitosis and apoptosis

an autosome and a sex chromosome

homologous pair of chromosomes and a sister chromatid

centromere and centriole

chromosome and chromatid

monozygotic and dizygotic twins

allele and gene

BIO130H1 Lecture Notes - Lecture 5: G2 Phase, G1 Phase, S Phase

BIO130H1 Full Course Notes

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