BIO130H1 Lecture Notes - Lecture 6: Dna Clamp, Dna Ligase, Primase

• School: UTSG
• Department: Biology
• Course: BIO130H1
• Professor: Melody Neumann

Document Summary

Interphase chromosomes are organized and are in specific/discrete locations in the nucleus (not a tangled mess of dna) Rules: dna is antiparallel, new dna is synthesized from 5" to 3", the template is read 3" to 5". How does dna replication proceed in bacteria: this style of replication only applies to circular genomes. 2 possibilities: (pulling apart 2 strands): random start. How many origins of replication: single in bacteria, multiple in eukaryotes. Procedure: separate dna strands, synthesize dna, proofread newly synthesized dna. Ingredients for synthesis: origin, primers, dntps, atp as an energy source, dna polymerase, accessory proteins. Binding of single- strand binding proteins: separates the strands by binding single stranded dna (ssdna, prevents strands from h- bonding. Rna primers made by primase (any type of primase is the same!) Nick sealing by dna ligase. The leading strand is synthesized continuously from one rna primer: true, false.