BIOB10H3 Lecture Notes - Lecture 9: Basal Body, Tau Protein, Immunoglobulin Light Chain

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23 Sep 2016
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What is in the cytosol: fluid matrix that surrounds all the organelles. What is in it: organelles, water / ions / sugars / atp, free ribosomes, lots of proteins. What is the signal for proteins to be targeted to the cytosol: no signal, synthesized on free ribosomes in cytosol. Movements of materials and organelles within cells: intracellular transport. Tubulin dimers: hollow tubular structures, found in almost all eukaryotic cells, dimerizing. Alpha-beta dimmers are the building blocks of a long polymer known as a protofilament: head to tail. Protofilament is asymmetric: alpha tubulin on one end. Minus - end (not affiliated with protein charge) Growth happens on the plus end: beta tubulin on another end. Plus + end (not affiliated with protein charge) Gets hydrolyzed slowly after polymerization occurs: characteristic of polarity. Each microtubule is composed of thirteen (13) protofilaments put together: aligned side by side, contains a hollow core, each protofilament has the same polarity, held by non-covalent interactions.

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