BIOA01H3 Lecture Notes - Lecture 25: Pyrimidine, Guanine, Purine

• School: UTSC
• Department: Biological Sciences
• Course: BIOA01H3
• Professor: Charmaine C Willia

Document Summary

How do we know dna is hereditary material: phage were allowed to infect bacteria grown on radiactive sulfur containing bacterial medium, phage were allowed to infect bacteria frown on radioactive phosphorus containing bacterial medium. If heridiatry material was protein, then s would be inserte din bacteria when phage affect its. Progeny from p containing dna did contain radioactivity: dna is heriditary material. Dna replication: template strand is 3 to 5, all of these enzymes only work 5-3 on replicated strand, the bond between hydroxyl and carbon is phosphdiester bond, pyrophosphase breakds down pyrophosphate. Leading strand moves towards rep fork, and 5 to 3. Laging strands moves away from rep fork, and primase adds primers allowing dna polymerase to (cid:272)o(cid:374)ti(cid:374)ue the sy(cid:374)thesis : dna polymerade -3 replaces all the rna primers with dna primers. In bacteria the replication forks meet eachother (because it has circular dna)