BIO380H5 Lecture Notes - Lecture 3: Petri Dish, Blastomere, Cytoskeleton
Document Summary
Imagine being a cell whose final job is being a very specialized cell i. The differentiation process will involve a lot of processing and replication to form specific cytoskeletal genes. Differentiation to start becoming i: maturation the decision making (to start transcribing, autonomous specification, experiment #1 i. ii. iii. Blastomeres destined to become ciliated cells are isolated from early embryo into a petri dish. They develop into the same ciliated cell types: early blastomeres are already specified. Removal of these cells: no tails muscles in larvae, conclusions, these cells are necessary for tail muscles, when cells are separated from the embryo, ex. A4. 2, a4. 4: each blastomere forms its respective cell types, conclusion, each blastomere was able to independently after separation form its respective type, we have specification but hesitant that this experiment provides evidence for autonomous specification iv. Ectopic misexpression of macho in other blastomeres: microinjection of macho mrna, ectopic muscle differentiation iv.
