BIO374H5 Lecture Notes - Lecture 6: Protein Structure Initiative, Bioreactor, National Institutes Of Health
Document Summary
The history is not that important for term test or final exam. The 3d structure of a protein is also important. Use of proteins in manufacturing is a time-tested technology: beer brewing and winemaking, cheese making. Recombinant dna technology made it possible to produce specific proteins on demand (give these examples on test: enzymes, hormones, antibodies. Biotech drugs and other medical applications: bioreactor control temperature, oxygen and ph to be able to produce the type of proteins, proteins can be inside or outside the cell. If extracellular, then we need to uptake the medium. Fda (food and drug administration) regulation in the us which is presented in the textbook. We want the protein to be 99. 9% pure based on fda. 60% or 50% pure: they may not function as intended by the manufacturer, may be contaminated with other proteins it may get people sick. Proteins that would be expected to be purified by recombinant methods: hiv treatment or digestive enzymes.
