BIO374H5 Lecture Notes - Lecture 7: High-Performance Liquid Chromatography, Two-Dimensional Gel Electrophoresis, Protein Purification
Document Summary
No need to know mechanical details on the test. Important to verify that target protein is not lost. Coomassie stain dye that combines with proteins so a colored band results. If not purified, we see a lot of bands. There are 4 purification steps (because there are 5 lanes) Primary antibody detect the protein that they"re able to detect. Secondary antibodies are applied to detect the primary antibodies that have binded. Excess secondary antibodies are washed off the membrane. Slides 4, 5, 16 (ex. of different types of biotech), 22 (there will be a question on this) Slides 12, 13 (r and s strain, be able to give explanation, how did you get the experimental results), 43 (gene expression),42 ,44, 46, 53 (epigenome know the concepts on the slide is good) Slides 4 (restriction enzymes, plasmid dna vectors), 7, 19 (mechanism behind it), 26 (differences between the two), 28, 38 (pcr), 51, 52, 53.
