BIO304H5 Lecture Notes - Lecture 2: Golgi'S Method, Heinrich Wilhelm Gottfried Von Waldeyer-Hartz, Reticular Theory

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The neuron doctrine: is the concept that the nervous system is made up of individual cells, a discovery due to neuro-anatomical work of ram n y cajal and later presented by h. waldeyer-hartz. We didn"t always know that neurons are cells. Schwann proposed that all organisms are composed of cells, but the nervous system was confusing to them because the relationship between dendrites + axons wasn"t clear (the relationship between large cell body and smaller features couldn"t be observed) Big development came from golgi who invented a staining technique called black reaction (golgi stain) This is a way to see nervous tissue on microscope. Staining is the way to see tiny structures on cells (like axons and dendrites) Golgi stain works like: stains some cells at random, dendrites are stained brown/black and this allows you to see connections between neurons. Golgi stains happens by putting potassium dichromate and silver nitrate on the nervous tissue (this crystallizes)