BIO200H5 Lecture Notes - Lecture 7: Headache, Cyclic Adenosine Monophosphate, Insulin Receptor
Document Summary
Record binding of drug to target = get kd (characterizes drug:target affinity, what doses to use in. Drugs with higher affinity for target = . Kd is a concentration specifically, a concentration of your drug acts as a baseline. Fbound = allows you to change the concentration of the free drug concentration. Kd looks as the interactions that create affinity between drug and receptor. it does not change when you add more drug you are just able to get to a saturation point sooner to determine kd sooner. Having identified what compound z binds to, you are attempting to determine some binding data between compound z and its target protein. you record the following information: Ex vivo (ex. / isolated tissues) experiments to measure . Dose and concentration are not the same thing. Of the drug that elicits half (50%) of the maximum inhibition.
