HSS 1100 Lecture Notes - Lecture 3: Hans Christian Gram, Gram Staining, Crystal Violet
26 Sep 2018
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HSS 1100 Lecture Notes - Lecture 2: Complement Membrane Attack Complex, Innate Immune System, Vacuum Cleaner
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HSS 1100 Lecture Notes - Lecture 3: Hans Christian Gram, Gram Staining, Crystal Violet
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Document Summary
Because from the isolation you can make a diagnosis. Short term vs long term: liquid nitrogen (-195 c, freezers, lyophilization (freeze-drying) Now that you have a pure culture. : colony morphology, cellular morphology. The microscope is your friend: resolving power (resolution) = ability to distinguish two closely located objects as separate, distinct entities. Generally 3 steps: make a smear on inoculating colony, fix dried smear by heat fix, stain with desired dye. Simple stain: single dye normally used, all organisms same colour, size, shape, number, arrangement, etc. Differential stain: two or more dyes, differences between microorganisms or parts of cells, acid fast, gram. Gram positive: thick peptidoglycan, no 2nd cell wall, not able to produce and liberate endotoxin. Gram negative: thin peptidoglycan, can produce and liberate endotoxin. Shape of bacteria related to peptidoglycan layer. Gram negative usually thinner than gram positive. Antibodies tagged with dyes are common (immunofluorescence microscopy)
