CHM 2354 Lecture Notes - Lecture 19: Capacity Factor, Elution, Theoretical Plate
25 May 2015
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Document Summary
Many chemical analyses/types of chromatography are required. Responds to potential impurities in the sample. Often necessary to first purify the compound of interest no remove interference this is called the separation step. Techniques available for chemical separations: extraction, distillation, precipitation, chromatography, others (filtration, centrifugation, etc. ) Biological samples are almost always composed of complex mixtures analysis of this composition and changes help in understanding disease and the development of treatments. Reference ranges have been created for common biological test like blood tests. A separation technique based on the different rates travel solutes through a system composed of two phases: a stationary and a mobile phase. Compounds are detected through emerging by changes in absorbance, current,voltage of. The compounds a and b will continually bind to the stationary phase and then dissociate to the mobile phase. Once you have that there is an equilibrium this is what chromatography is all about.
