BPS 4104 Lecture Notes - Lecture 4: Alternative Splicing, National Center For Biotechnology Information, Base Pair
27 Mar 2018
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Document Summary
Rna seq takes advantage of the poly a tail to sequence all of them. Impt to know which genes are silenced or expressed. Rna-seq then counts how many copies are present. Shorter reads and less accurate than sanger sequencing; originally called short sequence archive. Align the reads onto a known genome (cancer cell genome vs normal genome) Some are actively translated, have many ribosomes on it. Some mrna is actively transcribed but may not be actively translated. There"s a tunnel punching the mrna through, when mrna through tunnel, it"s not digested by rnases. Lots of areas mapped to certain gene means actively translated. Skipping of exons(exon 7 of usp4 is often skipped in mammals) Biosample (samn accession, e. g. , samn00004417, with links to bioprojects) Experiment (srx accession): info on what type of nucleic acid is extracted, enriched in which way, etc. , with links to run (srr accession)
