BIOL 202 Lecture Notes - Lecture 11: Forward Genetics, Northern Blot, Fluorescent Tag
Document Summary
Bioinformatics & genome annotation predicting genes and analysing the genome. Bioinformatics & comparative genomics within a genome (duplications and gene families), between organisms (synteny and core conserved genes) Functional genomics: transcriptomics ests, microarrays, rnaseq, chromatin immunoprecipitation chip. New: chapter 14, p519-525 + 527-532 + 536-539. Massively parallel sequencing: shorter runs (only a couple hundred nts at a time, can sequence >1m dna molecules in one expt. There are 3b bps in the human genome (approx. ) Isolate genomic dna from whatever tissue you want to sequence: sonicate to fragment the dna, sequence many millions of tiny dna fragments, powerful computer will take the millions of sequence reads and begin to analyse the sequences. It will look for overlaps to assemble a contig made up of multiple fragments: find enough contigs and the computer will find overlaps between them reassemble complete sequence. Bioinformatics and genome annotation: extraction of information content of the genome, identification of the full complement of encoded genes.
