BIO 4115 Lecture Notes - Lecture 2: Xanthine, Ancient Dna, Sickle-Cell Disease
12 Apr 2020
School
Department
Course
Professor
Document Summary
Damage sequencingancient dnachallenges contamination w microorganismsor modern dna few dnafragments. Chemical degradation deamination c to u which read as t. Basefrequency deamination c u creadas t s g xanthine small size. T subs are mostfrequent e occur mostly ends. Dnadamage vs seg error damaged g when exposedto stress w1 8 oxo dg affects only 1 base in the pairthere"s excess transversion errors from. G to t when mapped to ref genome. I 5 dnadamageintroduced treat samples w restorative enzymestr variantsfrom gto t to baselinelevels. Dnadamage wt c g g c g"t g"t s s z g"t c c s ligateadapter gps c g"t. Pa 3 z pag"t c c ps s meet gps c g"t. 3 t a a s ligateadapter s psa t. 3 pzt a a ps s meet s ps a t. T pa 3 s psa a t pe z. Diffpeoplerarelyhave same array distance bwth restrictionsites pop is polymorphic.
