BIO 3170 Lecture Notes - Lecture 4: Dna Replication, Okazaki Fragments, X-Ray Crystallography

33 views12 pages

Document Summary

Dna replication is described as semi-conservative: during replication each of the 2 old dna strands serves as a template for the formation of a complete new strand. Consequently, in each daughter cell, one dna strand corresponds to one of the dna strand of the mother cell while the second strand is newly synthesized. (different colors in the figure). After many generations, you will find one of the intact dna strands. Experimental evidence for semi-conservative dna replication (stahl & meselson, 1958: coli: grow e. coli in a broth containing n15, both strands were labelled with n15. Dna was centrifuged and separated according to density. Origi(cid:374)al (cid:271)roth is diluted allows one replication cycle (20 mins) All progeny will have one strand that is not radioactive n14. Dilute broth once again for another cycle of rep. Go onto cycle 3 most of progeny will contain non-labelled dna.

Get access

Grade+
$40 USD/m
Billed monthly
Grade+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
10 Verified Answers
Class+
$30 USD/m
Billed monthly
Class+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
7 Verified Answers

Related textbook solutions

Related Documents

Related Questions