BIO 3153 Lecture Notes - Lecture 3: Aequorin, Bioluminescence, X-Ray Crystallography
23 Sep 2016
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Slide 2: many other types, will discuss the two major types. Sem: (scanning) bombarding thick sample of tissue, electrons are bouncing off. (diffraction of electron from the sample) Resolution is higher than light microscope; 2000x the resolution. Can resolve objects 0. 1nm (1a), but usually 2 nm in biological preparations. Slide 3: tem: compared to lm, if we turn it over, we can make analogy to tem. Electrons are emitted at filament or cathode from electron gun instead of light source. Instead of lenses we have magnets; the magnets focus the path of electrons. Electron gets accelerated in high voltage in a vacuum, need vacuum because electron would then interact with the atmosphere. Sample might be stained to produce electron dense images. If something is dark (electron dense) light (electron lucent) Put grid on microscope, and pass electrons through the tissue.
